Laboratory Services

DOT maintains, and many States, strict guidelines regarding specimen processing. CMI tracks and monitors all testing to ensure the testing process meets these requirements.

Our contracted lab is SAMSHA certified.

Below is a listing of the substances that are tested under DOT requirements and our standard Non-DOT testing:

DOT Testing
Initial Test Analyte	Initial Test Cutoff Concentration	Confirmatory Test Analyte	Confirmatory Test Cutoff Concentration
Marijuna Metabolites	50 ng/mL	THCA¹	15 ng/mL
Cocaine Metabolites	150 ng/mL	Benzoylecgonine	100 ng/mL
Opiate Metabolites
Codeine/Morphine²	2000 ng/mL	Codeine	2000 ng/mL
		Morphine	2000 ng/mL
6-Acetylmorphine	10 ng/mL	6-Acetylmorphine	10 ng/mL
Phencyclidine	25 ng/mL	Phencyclidine	25 ng/mL
Amphetamines³
AMP/MAMP⁴	500 ng/mL	Amphetamine	250 ng/mL
		Methamphetamine⁵	250 ng/mL
MDMA⁶	500 ng/mL	MDMA	250 ng/mL
		MDA⁷	250 ng/mL
		MDEA⁸	250 ng/mL
¹Delta-9-tetrahydrocannabinol-9-carboxylic acid (THCA). ²Morphine is the target analyte for codeine/morphine testing. ³Either a single initial test kit or multiple initial test kits may be used provided the single test kit detects each target analyte independently at the specified cutoff. ⁴Methamphetamine is the target analyte for amphetamine/methamphetamine testing. ⁵To be reported positive for methamphetamine, a specimen must also contain amphetamine at a concentration equal to or greater than 100 ng/mL. ⁶Methylenedioxyethylamphetamine (MDEA). ⁷Methylenedioxyamphetamine (MDA). ⁸Methylenedioxymethamphetamine (MDMA).

Non-DOT Testing
Initial Test Analyte	Initial Test Cutoff Concentration	Confirmatory Test Analyte	Confirmatory Test Cutoff Concentration
Amphetamines	1000 ng/mL	Amphetamines	500 ng/mL
Barbiturates	300 ng/mL	Barbiturates	300 ng/mL
Benzodiazephones	300 ng/mL	Benzodiazephones	300 ng/mL
Cannabinoid Metabolites	50 ng/mL	Cannabinoid Metabolites	15 ng/mL
Cocaine Metabolites	300 ng/mL	Cocaine Metabolites	150 ng/mL
Opiates	300 ng/mL	Opiates	300 ng/mL
Phencyclidine (PCP)	25 ng/mL	Phencyclidine (PCP)	25 ng/mL
Methadone	300 ng/mL	Methadone	300 ng/mL
Methaqualone	300 ng/mL	Methaqualone	300 ng/mL
Propoxyphene	300 ng/mL	Propoxyphene	300 ng/mL

Initial Test: All specimens will be initially screened for the use of prohibited substances using an immunoassay test, which will eliminate negative urine specimens from further, or confirmatory, testing. Any specimen which resulted in a positive after the initial screening will be subject to confirmation screening using a more stringent and precise testing methodology.

Confirmatory Test: All specimens which are identified as positive on the initial test will be confirmed by a second screening independent from the initial screening which uses gas chromatography/mass spectrometry to confirm the initial positive test result.

Non-DOT Hair Testing
Initial Test Analyte	Initial Test Cutoff Concentration^*	Confirmatory Test Analyte	Confirmatory Test Cutoff Concentration^*
Amphetamines	5 ng/10 mL	Amphetamines	5 ng/10 mL
Cannabinoid Metabolites	2 ng/g	Cannabinoid Metabolites	1 pg/10 mL
Cocaine Metabolites	5 ng/10 mL	Cocaine Metabolites	5 ng/10 mL
Opiates	2 ng/10 mL	Opiates	2 ng/10 mL
Phencyclidine (PCP)	3 ng/10 mL	Phencyclidine (PCP)	3 ng/10 mL

Initial Test: All specimens will be initially screened for the use of prohibited substances using radioimmunoassay. RIAH® measures the drug molecules permanently entrapped in hair which were incorporated following ingestion. Any specimen which resulted in a positive after the initial screening will be subject to confirmation screening using a more stringent and precise testing methodology.

^*Cutoff Concentration levels are subject to changewith scientific advancements.

DOT Testing

Non-DOT Testing

Non-DOT Hair Testing